SUBMERGED CULTURE OF MAMMALIAN CELLS: THE FIVE LITER FERMENTOR,
نویسندگان
چکیده
منابع مشابه
The propagation of mammalian cells in a 20-liter stainless steel fermentor.
The propagation of mammalian cells in stainless steel fermentors of a type used in antibiotic fermentations represents a marked departure from the techniques employed in classical tissue culture studies. Since the pioneer work of Harrison (1907), in which he demonstrated the outgrowth of cells from nerve tissue maintained in vitro, many techniques for the in vitro culture of cells have been dev...
متن کاملAn Improved Laboratory-Scale Fermentor for Submerged Culture Investigations.
A small laboratory fermentor which employs mechanical agitation for the dispersion of air introduced under pressure has previously been described by Feustel and Humfeld (1946). This fermentor has an operating capacity of 500 to 2,000 ml and has been found very useful for small-scale, yeast-culturing investigations, as well as for studies on the production of subtilin activity by Bacillus subtil...
متن کاملThe Propagation of Mammalian Cells in a 20 - Liter
The propagation of mammalian cells in stainless steel fermentors of a type used in antibiotic fermentations represents a marked departure from the techniques employed in classical tissue culture studies. Since the pioneer work of Harrison (1907), in which he demonstrated the outgrowth of cells from nerve tissue maintained in vitro, many techniques for the in vitro culture of cells have been dev...
متن کاملGrowth kinetic models of five species of Lactobacilli and lactose consumption in batch submerged culture
Kinetic behaviors of five Lactobacillus strains were investigated with Contois and Exponential models. Awareness of kinetic behavior of microorganisms is essential for their industrial process design and scale up. The consistency of experimental data was evaluated using Excel software. L. bulgaricus was introduced as the most efficient strain with the highest biomass and lactic acid yield of 0....
متن کاملCatalase Requirement for Mammalian Cells in Culture
The studies of Puck and his coworkers (1) have made possible the growth of single mammalian cells by a procedure comparable to the plating technique of microbiology. Knowledge of the growth requirements peculiar to single cells (2) or small numbers of cells, however, is essentially lacking. Although the mutually advantageous interaction of cells growing close together was indicated by Sanford e...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1957
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.74.6.768-774.1957